Journal: Burns & Trauma

Article Title: TAK1 activates PANoptosis through the NF-κB signalling pathway to delay diabetic wound healing

doi: 10.1093/burnst/tkag001

Figure Lengend Snippet: PANoptosis occurs in diabetic wounds and cells in a hyperglycaemic environment. ( a , d) TUNEL staining and quantitative statistical analysis of skin tissue samples. (b, e) EthD-III staining and quantitative statistical analysis of skin tissue samples. ( c , f ) PI staining and quantitative statistical analysis of skin tissue samples. ( g ) Western blot analysis showing changes in the expression of necroptosis-, pyroptosis-, and apoptosis-related proteins at different time points in DW and NW tissue. ( h , i ) Quantitative statistics of necroptosis-related protein expression levels. ( j , k ) Quantitative statistics of pyroptosis-related protein expression levels. ( l , m ) Quantitative statistics of apoptosis-related protein expression levels. ( n , q ) TUNEL staining and quantitative statistical analysis of skin tissue samples. ( o , r ) EthD-III staining of skin tissues and quantitative statistical analysis. p, s. PI staining of skin tissues and quantitative statistical analysis. Scale bars: 50 μm. * P < 0.05, * * P < 0.01, * * * P <0.001 versus NG. # P < 0.05, # # P < 0.01, # # # P < 0.001 versus HG. DW diabetic wound, NW normal wound, NG normal glucose. HG high glucose, TUNEL terminal deoxynucleotidyl transferase dUTP nick end labelling. EthD-III ethidium homodimer III, PI propidium iodide

Article Snippet: EthD-III (Biotium, Fremont, CA, USA; Cat# 40050) is a fluorescent dye that stains dead cells with compromised membranes and is commonly used to detect pyroptosis [ ].

Techniques: TUNEL Assay, Staining, Western Blot, Expressing

Journal: Burns & Trauma

Article Title: TAK1 activates PANoptosis through the NF-κB signalling pathway to delay diabetic wound healing

doi: 10.1093/burnst/tkag001

Figure Lengend Snippet: Regulatory role of TAK1 in PANoptosis under HG conditions. ( a , d ) TUNEL staining and quantitative statistical analysis of skin tissue samples (scale: 20 μm). ( b , e ) EthD-III staining and quantitative statistical analysis of skin tissue samples (scale: 20 μm). ( c , f ) PI staining and quantitative statistical analysis of skin tissue samples (scale: 20 μm). ( g – n ) Protein expression levels and quantitative analysis of p-RIPK1, RIPK1, cleaved caspase-3, NLRP3, ZBP1, ASC, and AIM2 expression. ( o ) Representative images of cells after coimmunostaining for RIPK1 and FADD (scale: 10 μm). ( p ) Immunoprecipitation analysis of the interaction between RIPK1 and FADD. * P < 0.05, * * P < 0.01, * * * P < 0.001. NG normal glucose, HG high glucose, TUNEL terminal deoxynucleotidyl transferase dUTP nick end labelling, EthD-III ethidium homodimer III, PI propidium iodide

Article Snippet: EthD-III (Biotium, Fremont, CA, USA; Cat# 40050) is a fluorescent dye that stains dead cells with compromised membranes and is commonly used to detect pyroptosis [ ].

Techniques: TUNEL Assay, Staining, Expressing, Immunoprecipitation

Journal: Burns & Trauma

Article Title: TAK1 activates PANoptosis through the NF-κB signalling pathway to delay diabetic wound healing

doi: 10.1093/burnst/tkag001

Figure Lengend Snippet: TAK1 induces PANoptosis through NF-κB signalling under HG conditions. ( a , c ) TUNEL staining of cells and quantitative statistical analysis, scale bars: 50 μm. ( b , d ) EthD-III staining of cells and quantitative statistical analysis, scale bars: 50 μm. ( e , g ) PI staining of cells and quantitative statistical analysis, scale bars: 50 μm. ( f , h – j ) Expression and quantitative analysis of p-RIPK1, RIPK1, cleaved caspase-3, and cleaved caspase-1 protein levels. ( k – n ) IL-1β, IL-6, IL-18, and TNF-α levels measured by ELISA. * * P < 0.01, * * * P < 0.001 versus NG. # P <0.05, # # P <0.01, # # # P < 0.001 versus HG. & P < .0.05, && P < 0.01 versus HG + si- TAK1 + BAY 11–7082. NG normal glucose, HG high glucose, TUNEL terminal deoxynucleotidyl transferase dUTP nick end labelling, EthD-III ethidium homodimer III, PI propidium iodide

Article Snippet: EthD-III (Biotium, Fremont, CA, USA; Cat# 40050) is a fluorescent dye that stains dead cells with compromised membranes and is commonly used to detect pyroptosis [ ].

Techniques: TUNEL Assay, Staining, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Burns & Trauma

Article Title: TAK1 activates PANoptosis through the NF-κB signalling pathway to delay diabetic wound healing

doi: 10.1093/burnst/tkag001

Figure Lengend Snippet: Effects of TAK1 inhibition on PANoptosis markers and cell death in HG conditions. ( a , c , d ) Expression and quantitative analysis of p-IKBα/IKBα and p-P65/P65 protein levels. ( b , e – h ) Expression and quantitative analysis of cleaved caspase-3, cleaved caspase-1, p-RIPK1/RIPK1, and ZBP1 protein levels. ( i , l ) TUNEL staining of cells and quantitative statistical analysis, scale bars: 50 μm. ( j , m ) EthD-III staining of cells and quantitative statistical analysis, scale bars: 50 μm. ( k , n ) PI staining of cells and quantitative statistical analysis, scale bars: 50 μm. * * * P < 0.001 versus NG. # P < 0.05, # # P < 0.01, # # # P < 0.001 versus HG. & P < 0.05, && P < 0.01 versus HG + si- TAK1 + IKKβ-OE. NG normal glucose, HG high glucose, TUNEL terminal deoxynucleotidyl transferase dUTP nick end labelling, EthD-II , ethidium homodimer III, PI propidium iodide

Article Snippet: EthD-III (Biotium, Fremont, CA, USA; Cat# 40050) is a fluorescent dye that stains dead cells with compromised membranes and is commonly used to detect pyroptosis [ ].

Techniques: Inhibition, Expressing, TUNEL Assay, Staining

Journal: Scientific Reports

Article Title: A perfusable decellularized plant-based air-liquid-interface-on-a-chip for investigating inorganic dust aerosol exposure

doi: 10.1038/s41598-025-33930-7

Figure Lengend Snippet: Diagram of air–liquid-interface platform for dust exposure. ( A ) Stepwise experimental timeline of the decellularized plant-based ALI model and dust exposure. ( B ) 3D design of the platform, which provides an enclosed chamber for accommodating spinach leaves. ( C ) The macroscopic image of the recellularized spinach integrated into the air–liquid-interface platform. Live/Dead fluorescence images (Calcein-AM = green, EthD-1 = red) of recellularized leaves (Left and right panels correspond to images taken from different focal planes representing the apical epithelial surface and the vascular endothelial layer, respectively). ( D ) Quantitative cell viability of BEAS-2B and HUVECs analyzed using ImageJ software (NIH, USA).

Article Snippet: Cell viability within the recellularized spinach scaffolds was evaluated using a Calcein-AM/Ethidium homodimer-1 (EthD-1) Live/Dead Viability/Cytotoxicity Kit (Biotium, USA) following the manufacturer’s protocol.

Techniques: Fluorescence, Software